                         SEQUENCE LISTING
<110>  Bayer CropScience GmbH
<120>  TRANSFORMED PLANT EXPRESSING A MUTANSUCRASE AND SYNTHESIZING A MODIFIED STARCH
<130>  BCS 04-5011-PCT
<160>  4     
<170>  PatentIn version 3.1
<210>  1
<211>  4794
<212>  DNA
<213>  Streptococcus downei
<220>
<221>  sig_peptide
<222>  (1)..(114)
<223>  sequence encoding mutansucrase signal peptide for secretion

<220>
<221>  CDS
<222>  (1)..(4791)
<223>  

<220>
<221>  misc_feature
<222>  (3154)..(4197)
<223>  C-terminal (glucan binding) domain of gene encoding mutansucrase 

<220>
<221>  misc_feature
<222>  (115)..(438)
<223>  variable region of gene encoding mutansucrase

<220>
<221>  misc_feature
<222>  (439)..(3153)
<223>  active core region of gene encoding mutansucrase

<400>  1
atg gag aag aat gaa cgt ttt aag atg cat aag gtc aaa aag aga tgg       48
Met Glu Lys Asn Glu Arg Phe Lys Met His Lys Val Lys Lys Arg Trp         
1               5                   10                  15              

gtg act atc tca gtt gca tct gcc act atg tta gct tca gct ctc ggt       96
Val Thr Ile Ser Val Ala Ser Ala Thr Met Leu Ala Ser Ala Leu Gly         
            20                  25                  30                  

gct tca gtt gct agc gca gat act gaa act gtt agc gaa gac agc aac      144
Ala Ser Val Ala Ser Ala Asp Thr Glu Thr Val Ser Glu Asp Ser Asn         
        35                  40                  45                      

caa gca gtc ttg acg gct gac caa acg act acc aac caa gat act gag      192
Gln Ala Val Leu Thr Ala Asp Gln Thr Thr Thr Asn Gln Asp Thr Glu         
    50                  55                  60                          

caa act tct gtt gca gcg aca gct aca tca gaa cag tct gct tca act      240
Gln Thr Ser Val Ala Ala Thr Ala Thr Ser Glu Gln Ser Ala Ser Thr         
65                  70                  75                  80          

gat gca gca aca gat caa gca tca gca aca gat caa gca tca gca gca      288
Asp Ala Ala Thr Asp Gln Ala Ser Ala Thr Asp Gln Ala Ser Ala Ala         
                85                  90                  95              

gag caa act caa gga aca aca gct agc aca gac acg gca gct caa aca      336
Glu Gln Thr Gln Gly Thr Thr Ala Ser Thr Asp Thr Ala Ala Gln Thr         
            100                 105                 110                 

acc aca aat gct aat gaa gct aag tgg gtt ccg act gaa aat gag aac      384
Thr Thr Asn Ala Asn Glu Ala Lys Trp Val Pro Thr Glu Asn Glu Asn         
        115                 120                 125                     

caa gtt ttt aca gat gag atg tta gca gaa gcc aag aat gtg gct act      432
Gln Val Phe Thr Asp Glu Met Leu Ala Glu Ala Lys Asn Val Ala Thr         
    130                 135                 140                         

gct gaa tct aat tca att cca tca gac ttg gct aaa atg tca aat gtt      480
Ala Glu Ser Asn Ser Ile Pro Ser Asp Leu Ala Lys Met Ser Asn Val         
145                 150                 155                 160         

aag cag gtt gac ggt aaa tat tat tac tac gat caa gac ggc aac gtt      528
Lys Gln Val Asp Gly Lys Tyr Tyr Tyr Tyr Asp Gln Asp Gly Asn Val         
                165                 170                 175             

aag aag aac ttt gct gtt agc gtt ggt gag aag atc tat tac ttt gat      576
Lys Lys Asn Phe Ala Val Ser Val Gly Glu Lys Ile Tyr Tyr Phe Asp         
            180                 185                 190                 

gaa act ggc gct tac aaa gac act agc aag gta gaa gcg gat aaa tca      624
Glu Thr Gly Ala Tyr Lys Asp Thr Ser Lys Val Glu Ala Asp Lys Ser         
        195                 200                 205                     

ggt tct gat atc agc aaa gaa gaa aca acc ttt gct gct aac aac cgt      672
Gly Ser Asp Ile Ser Lys Glu Glu Thr Thr Phe Ala Ala Asn Asn Arg         
    210                 215                 220                         

gct tac agt acc tca gct gaa aac ttt gaa gcc att gat aac tac ttg      720
Ala Tyr Ser Thr Ser Ala Glu Asn Phe Glu Ala Ile Asp Asn Tyr Leu         
225                 230                 235                 240         

aca gct gac tca tgg tac cgt cca aag tct atc ctc aag gat ggt aag      768
Thr Ala Asp Ser Trp Tyr Arg Pro Lys Ser Ile Leu Lys Asp Gly Lys         
                245                 250                 255             

act tgg aca gaa tca agc aag gat gac ttc cgt ccg cta ttg atg gct      816
Thr Trp Thr Glu Ser Ser Lys Asp Asp Phe Arg Pro Leu Leu Met Ala         
            260                 265                 270                 

tgg tgg cca gat acc gaa acc aaa cgc aac tat gtt aac tac atg aac      864
Trp Trp Pro Asp Thr Glu Thr Lys Arg Asn Tyr Val Asn Tyr Met Asn         
        275                 280                 285                     

aag gtt gtt ggt atc gat aaa acc tat acc gct gaa aca agc cag gct      912
Lys Val Val Gly Ile Asp Lys Thr Tyr Thr Ala Glu Thr Ser Gln Ala         
    290                 295                 300                         

gac ctg aca gca gca gct gaa ctg gtt caa gcc cgt atc gaa caa aag      960
Asp Leu Thr Ala Ala Ala Glu Leu Val Gln Ala Arg Ile Glu Gln Lys         
305                 310                 315                 320         

att aca act gaa caa aat acc aaa tgg ttg cgt gaa gct atc tca gct     1008
Ile Thr Thr Glu Gln Asn Thr Lys Trp Leu Arg Glu Ala Ile Ser Ala         
                325                 330                 335             

ttt gtt aaa act caa cca caa tgg aat ggt gaa agc gaa aag cca tac     1056
Phe Val Lys Thr Gln Pro Gln Trp Asn Gly Glu Ser Glu Lys Pro Tyr         
            340                 345                 350                 

gat gac cac ttg caa aat ggt gcc ctc aag ttc gat aac caa tct gat     1104
Asp Asp His Leu Gln Asn Gly Ala Leu Lys Phe Asp Asn Gln Ser Asp         
        355                 360                 365                     

ttg aca cca gat acg caa tcg aac tat cgt ttg ctc aat cgc aca cca     1152
Leu Thr Pro Asp Thr Gln Ser Asn Tyr Arg Leu Leu Asn Arg Thr Pro         
    370                 375                 380                         

act aac caa act ggt tcc ctg gac tct cgt ttc acc tac aat gct aac     1200
Thr Asn Gln Thr Gly Ser Leu Asp Ser Arg Phe Thr Tyr Asn Ala Asn         
385                 390                 395                 400         

gac ccg tta ggt ggt tat gag ttg ctt ctg gct aac gac gtg gat aac     1248
Asp Pro Leu Gly Gly Tyr Glu Leu Leu Leu Ala Asn Asp Val Asp Asn         
                405                 410                 415             

tct aat ccc atc gtt caa gca gag caa ctc aac tgg ctg cat tac ctg     1296
Ser Asn Pro Ile Val Gln Ala Glu Gln Leu Asn Trp Leu His Tyr Leu         
            420                 425                 430                 

ctc aac ttc ggt act atc tat gct aaa gat gcg gat gct aac ttt gac     1344
Leu Asn Phe Gly Thr Ile Tyr Ala Lys Asp Ala Asp Ala Asn Phe Asp         
        435                 440                 445                     

tct atc cgt gtt gat gcg gta gat aat gtc gat gct gac ctt ctg caa     1392
Ser Ile Arg Val Asp Ala Val Asp Asn Val Asp Ala Asp Leu Leu Gln         
    450                 455                 460                         

atc tct agt gat tac ctt aag gca gct tac ggt atc gat aaa aac aac     1440
Ile Ser Ser Asp Tyr Leu Lys Ala Ala Tyr Gly Ile Asp Lys Asn Asn         
465                 470                 475                 480         

aaa aat gct aat aac cac gtt tct atc gta gaa gca tgg agc gac aac     1488
Lys Asn Ala Asn Asn His Val Ser Ile Val Glu Ala Trp Ser Asp Asn         
                485                 490                 495             

gat acc cct tat ctc cat gat gat ggc gac aac ctc atg aac atg gac     1536
Asp Thr Pro Tyr Leu His Asp Asp Gly Asp Asn Leu Met Asn Met Asp         
            500                 505                 510                 

aac aag ttc cgt ttg tct atg ctt tgg tct ttg gct aaa cca ttg gac     1584
Asn Lys Phe Arg Leu Ser Met Leu Trp Ser Leu Ala Lys Pro Leu Asp         
        515                 520                 525                     

aaa cgt tct ggc ttg aat ccc ctc atc cac aac agt ctg gtt gac cgt     1632
Lys Arg Ser Gly Leu Asn Pro Leu Ile His Asn Ser Leu Val Asp Arg         
    530                 535                 540                         

gaa gtg gat gac cgt gaa gtt gaa acc gtt cca agt tac agc ttt gcc     1680
Glu Val Asp Asp Arg Glu Val Glu Thr Val Pro Ser Tyr Ser Phe Ala         
545                 550                 555                 560         

cgt gct cac gat agc gaa gta caa gac ctg att cgt gac atc atc aag     1728
Arg Ala His Asp Ser Glu Val Gln Asp Leu Ile Arg Asp Ile Ile Lys         
                565                 570                 575             

gct gaa att aat cca aat gca ttt ggt tat tca ttt act caa gac gaa     1776
Ala Glu Ile Asn Pro Asn Ala Phe Gly Tyr Ser Phe Thr Gln Asp Glu         
            580                 585                 590                 

atc gac caa gcc ttc aag att tac aac gaa gac ctc aag aag acc gat     1824
Ile Asp Gln Ala Phe Lys Ile Tyr Asn Glu Asp Leu Lys Lys Thr Asp         
        595                 600                 605                     

aag aaa tac act cac tac aat gtg ccg ctt tct tat acc ttg ctt ctg     1872
Lys Lys Tyr Thr His Tyr Asn Val Pro Leu Ser Tyr Thr Leu Leu Leu         
    610                 615                 620                         

act aac aag ggt tcg att cct cgc gtc tat tat gga gat atg ttc acc     1920
Thr Asn Lys Gly Ser Ile Pro Arg Val Tyr Tyr Gly Asp Met Phe Thr         
625                 630                 635                 640         

gat gat ggt caa tac atg gcc aac aag act gtg aac tac gat gct atc     1968
Asp Asp Gly Gln Tyr Met Ala Asn Lys Thr Val Asn Tyr Asp Ala Ile         
                645                 650                 655             

gaa tct ctg ctg aaa gcc cgt atg aag tac gtt gct ggt ggt caa gct     2016
Glu Ser Leu Leu Lys Ala Arg Met Lys Tyr Val Ala Gly Gly Gln Ala         
            660                 665                 670                 

atg caa aat tac caa atc ggt aat ggc gaa atc ttg act tct gtc cgt     2064
Met Gln Asn Tyr Gln Ile Gly Asn Gly Glu Ile Leu Thr Ser Val Arg         
        675                 680                 685                     

tat ggt aag ggt gcc ctt aaa caa agc gat aag ggt gat gcg aca act     2112
Tyr Gly Lys Gly Ala Leu Lys Gln Ser Asp Lys Gly Asp Ala Thr Thr         
    690                 695                 700                         

cgt acg tca ggt gtc ggc gtt gtt atg gga aac caa ccc aac ttt agc     2160
Arg Thr Ser Gly Val Gly Val Val Met Gly Asn Gln Pro Asn Phe Ser         
705                 710                 715                 720         

ttg gat gga aag gtt gta gcc ctc aac atg ggt gct gcc cac gct aac     2208
Leu Asp Gly Lys Val Val Ala Leu Asn Met Gly Ala Ala His Ala Asn         
                725                 730                 735             

caa gaa tac cgt gct ctt atg gta tca act aaa gac ggt gtt gca acc     2256
Gln Glu Tyr Arg Ala Leu Met Val Ser Thr Lys Asp Gly Val Ala Thr         
            740                 745                 750                 

tat gct aca gat gct gat gct agc aag gct ggt ctg gtt aag cgc aca     2304
Tyr Ala Thr Asp Ala Asp Ala Ser Lys Ala Gly Leu Val Lys Arg Thr         
        755                 760                 765                     

gat gaa aat ggt tac ctc tac ttc ttg aac gac gat ctc aag ggg gtt     2352
Asp Glu Asn Gly Tyr Leu Tyr Phe Leu Asn Asp Asp Leu Lys Gly Val         
    770                 775                 780                         

gct aac cct cag gtt tct ggt ttc ctt caa gtc tgg gta cca gtg gga     2400
Ala Asn Pro Gln Val Ser Gly Phe Leu Gln Val Trp Val Pro Val Gly         
785                 790                 795                 800         

gca gca gat gac caa gat att cgt gta gca gct agc gat aca gca agt     2448
Ala Ala Asp Asp Gln Asp Ile Arg Val Ala Ala Ser Asp Thr Ala Ser         
                805                 810                 815             

acc gat gga aaa tca ctc cat caa gat gct gcc atg gac tct cgc gtc     2496
Thr Asp Gly Lys Ser Leu His Gln Asp Ala Ala Met Asp Ser Arg Val         
            820                 825                 830                 

atg ttt gaa ggt ttc tct aac ttc caa tct ttt gcg aca aaa gaa gaa     2544
Met Phe Glu Gly Phe Ser Asn Phe Gln Ser Phe Ala Thr Lys Glu Glu         
        835                 840                 845                     

gag tat acc aat gtt gtt att gct aac aat gtt gat aaa ttt gtt tca     2592
Glu Tyr Thr Asn Val Val Ile Ala Asn Asn Val Asp Lys Phe Val Ser         
    850                 855                 860                         

tgg gga atc act gac ttt gaa atg gct cct cag tat gtc tca tct act     2640
Trp Gly Ile Thr Asp Phe Glu Met Ala Pro Gln Tyr Val Ser Ser Thr         
865                 870                 875                 880         

gac ggt cag ttc ctt gat tct gtc att caa aat ggt tat gcc ttt acc     2688
Asp Gly Gln Phe Leu Asp Ser Val Ile Gln Asn Gly Tyr Ala Phe Thr         
                885                 890                 895             

gac cgt tat gac ttg ggt atg tct aaa gca aac aag tat ggt aca gcc     2736
Asp Arg Tyr Asp Leu Gly Met Ser Lys Ala Asn Lys Tyr Gly Thr Ala         
            900                 905                 910                 

gac caa ttg gtt aag gct atc aag gct ctc cat gct aaa ggc ctg aag     2784
Asp Gln Leu Val Lys Ala Ile Lys Ala Leu His Ala Lys Gly Leu Lys         
        915                 920                 925                     

gtt atg gca gac tgg gtt cca gac caa atg tac acc ttc cct aaa caa     2832
Val Met Ala Asp Trp Val Pro Asp Gln Met Tyr Thr Phe Pro Lys Gln         
    930                 935                 940                         

gaa gtg gtc act gtt act cgg aca gat aag ttt ggc aaa cca atc gca     2880
Glu Val Val Thr Val Thr Arg Thr Asp Lys Phe Gly Lys Pro Ile Ala         
945                 950                 955                 960         

gga agc caa att aat cac agt ctc tac gta aca gat aca aag agc tct     2928
Gly Ser Gln Ile Asn His Ser Leu Tyr Val Thr Asp Thr Lys Ser Ser         
                965                 970                 975             

ggt gat gac tat caa gct aaa tac ggc ggt gcc ttc ctt gac gaa tta     2976
Gly Asp Asp Tyr Gln Ala Lys Tyr Gly Gly Ala Phe Leu Asp Glu Leu         
            980                 985                 990                 

aag gaa aaa tat cca gaa ctc ttt  acc aag aag caa atc  tct acc ggc   3024
Lys Glu Lys Tyr Pro Glu Leu Phe  Thr Lys Lys Gln Ile  Ser Thr Gly       
        995                 1000                 1005                   

caa gcc  ata gat cca tct gtt  aag att aaa caa tgg  tct gct aag      3069
Gln Ala  Ile Asp Pro Ser Val  Lys Ile Lys Gln Trp  Ser Ala Lys          
    1010                 1015                 1020                      

tac ttc  aac ggt agc aat atc  ctt ggc cgg ggt gcc  gat tat gtc      3114
Tyr Phe  Asn Gly Ser Asn Ile  Leu Gly Arg Gly Ala  Asp Tyr Val          
    1025                 1030                 1035                      

ctc agc  gac caa gca agc aac  aag tac ctc aat gtt  tca gat gat      3159
Leu Ser  Asp Gln Ala Ser Asn  Lys Tyr Leu Asn Val  Ser Asp Asp          
    1040                 1045                 1050                      

aaa ctc  ttc ttg cca aaa act  ctc cta ggt caa gtc  gta gaa tca      3204
Lys Leu  Phe Leu Pro Lys Thr  Leu Leu Gly Gln Val  Val Glu Ser          
    1055                 1060                 1065                      

ggt atc  cgc ttt gat gga act  ggt tat gtc tac aac  tca agc aca      3249
Gly Ile  Arg Phe Asp Gly Thr  Gly Tyr Val Tyr Asn  Ser Ser Thr          
    1070                 1075                 1080                      

aca ggt  gaa aag gta acc gat  agc ttt att act gaa  gct ggt aat      3294
Thr Gly  Glu Lys Val Thr Asp  Ser Phe Ile Thr Glu  Ala Gly Asn          
    1085                 1090                 1095                      

ctt tac  tac ttt ggc caa gat  ggt tac atg gtg act  ggt gcc caa      3339
Leu Tyr  Tyr Phe Gly Gln Asp  Gly Tyr Met Val Thr  Gly Ala Gln          
    1100                 1105                 1110                      

aac atc  aag gga tct aac tat  tac ttc ctg gct aat  ggt gct gcc      3384
Asn Ile  Lys Gly Ser Asn Tyr  Tyr Phe Leu Ala Asn  Gly Ala Ala          
    1115                 1120                 1125                      

ctt cgc  aat aca gtt tat act  gat gcc caa ggt caa  aac cat tac      3429
Leu Arg  Asn Thr Val Tyr Thr  Asp Ala Gln Gly Gln  Asn His Tyr          
    1130                 1135                 1140                      

tat ggc  aac gac ggt aaa cgt  tac gaa aat ggt tac  caa caa ttt      3474
Tyr Gly  Asn Asp Gly Lys Arg  Tyr Glu Asn Gly Tyr  Gln Gln Phe          
    1145                 1150                 1155                      

ggt aat  gac agc tgg cgt tac  ttc aag aat ggt gtc  atg gca ctt      3519
Gly Asn  Asp Ser Trp Arg Tyr  Phe Lys Asn Gly Val  Met Ala Leu          
    1160                 1165                 1170                      

ggc ctg  aca acc gtt gat ggt  cac gtt caa tac ttt  gat aaa gat      3564
Gly Leu  Thr Thr Val Asp Gly  His Val Gln Tyr Phe  Asp Lys Asp          
    1175                 1180                 1185                      

ggt gtt  caa gct aag gat aag  att att gtc acc cgt  gat ggt aag      3609
Gly Val  Gln Ala Lys Asp Lys  Ile Ile Val Thr Arg  Asp Gly Lys          
    1190                 1195                 1200                      

gtt cgt  tac ttc gac caa cat  aat gga aat gct gta  acc aat acc      3654
Val Arg  Tyr Phe Asp Gln His  Asn Gly Asn Ala Val  Thr Asn Thr          
    1205                 1210                 1215                      

ttc gtc  gct gac aag act ggt  cac tgg tac tat cta  ggt aaa gat      3699
Phe Val  Ala Asp Lys Thr Gly  His Trp Tyr Tyr Leu  Gly Lys Asp          
    1220                 1225                 1230                      

ggt gtc  gct gtt acc ggt gct  caa act gtt ggt aaa  caa cac ctt      3744
Gly Val  Ala Val Thr Gly Ala  Gln Thr Val Gly Lys  Gln His Leu          
    1235                 1240                 1245                      

tac ttc  gaa gcc aat ggt caa  caa gtt aag ggt gac  ttt gtc aca      3789
Tyr Phe  Glu Ala Asn Gly Gln  Gln Val Lys Gly Asp  Phe Val Thr          
    1250                 1255                 1260                      

gcc aaa  gat ggt aaa ctt tac  ttc tac gat gtt gac  tct ggt gac      3834
Ala Lys  Asp Gly Lys Leu Tyr  Phe Tyr Asp Val Asp  Ser Gly Asp          
    1265                 1270                 1275                      

atg tgg  acc aat acc ttc att  gaa gac aag gca ggc  aac tgg ttc      3879
Met Trp  Thr Asn Thr Phe Ile  Glu Asp Lys Ala Gly  Asn Trp Phe          
    1280                 1285                 1290                      

tat ctt  ggt aaa gat ggc gca  gct gtc aca ggt gct  caa acc atc      3924
Tyr Leu  Gly Lys Asp Gly Ala  Ala Val Thr Gly Ala  Gln Thr Ile          
    1295                 1300                 1305                      

aag ggc  caa aaa ctt tac ttc  aag gct aac ggc caa  caa gtt aag      3969
Lys Gly  Gln Lys Leu Tyr Phe  Lys Ala Asn Gly Gln  Gln Val Lys          
    1310                 1315                 1320                      

ggt gat  atc gtc aag gat gcc  gat ggt aag att cgt  tac tac gat      4014
Gly Asp  Ile Val Lys Asp Ala  Asp Gly Lys Ile Arg  Tyr Tyr Asp          
    1325                 1330                 1335                      

gcc caa  act ggt gaa caa gtc  ttt aat aag tct gta  agt gtt aac      4059
Ala Gln  Thr Gly Glu Gln Val  Phe Asn Lys Ser Val  Ser Val Asn          
    1340                 1345                 1350                      

ggt aag  act tac tac ttc ggt  agt gat gga act gct  caa act cag      4104
Gly Lys  Thr Tyr Tyr Phe Gly  Ser Asp Gly Thr Ala  Gln Thr Gln          
    1355                 1360                 1365                      

gct aat  cca aag ggt caa acc  ttt aag gat ggt tct  gga gtt ctt      4149
Ala Asn  Pro Lys Gly Gln Thr  Phe Lys Asp Gly Ser  Gly Val Leu          
    1370                 1375                 1380                      

cgt ttc  tac aat ctt gaa ggt  cag tat gta tca ggt  agt gga tgg      4194
Arg Phe  Tyr Asn Leu Glu Gly  Gln Tyr Val Ser Gly  Ser Gly Trp          
    1385                 1390                 1395                      

tat gaa  aca gca gag cac gaa  tgg gtt tat gtt aaa  tct ggt aag      4239
Tyr Glu  Thr Ala Glu His Glu  Trp Val Tyr Val Lys  Ser Gly Lys          
    1400                 1405                 1410                      

gta ttg  act ggt gct caa acc  att gga aat caa cga  gtt tac ttc      4284
Val Leu  Thr Gly Ala Gln Thr  Ile Gly Asn Gln Arg  Val Tyr Phe          
    1415                 1420                 1425                      

aag gat  aat ggc cat caa gtc  aaa ggt caa ttg gta  act ggt aac      4329
Lys Asp  Asn Gly His Gln Val  Lys Gly Gln Leu Val  Thr Gly Asn          
    1430                 1435                 1440                      

gat ggt  aag ctt cgc tac tat  gat gcc aac tct ggc  gac cag gct      4374
Asp Gly  Lys Leu Arg Tyr Tyr  Asp Ala Asn Ser Gly  Asp Gln Ala          
    1445                 1450                 1455                      

ttc aac  aag tct gtg act gtt  aat ggc aaa act tac  tac ttc ggt      4419
Phe Asn  Lys Ser Val Thr Val  Asn Gly Lys Thr Tyr  Tyr Phe Gly          
    1460                 1465                 1470                      

agt gat  gga act gct caa act  cag gct aat cca aag  ggt caa acc      4464
Ser Asp  Gly Thr Ala Gln Thr  Gln Ala Asn Pro Lys  Gly Gln Thr          
    1475                 1480                 1485                      

ttt aag  gat ggt tct gga gtt  ctt cgt ttc tac aat  ctt gaa ggt      4509
Phe Lys  Asp Gly Ser Gly Val  Leu Arg Phe Tyr Asn  Leu Glu Gly          
    1490                 1495                 1500                      

cag tat  gta tca ggt agt gga  tgg tat aaa aat gcc  caa ggt caa      4554
Gln Tyr  Val Ser Gly Ser Gly  Trp Tyr Lys Asn Ala  Gln Gly Gln          
    1505                 1510                 1515                      

tgg ctt  tac gtt aaa gac gga  aaa gta ttg act ggc  ctg cag aca      4599
Trp Leu  Tyr Val Lys Asp Gly  Lys Val Leu Thr Gly  Leu Gln Thr          
    1520                 1525                 1530                      

gta ggt  aac caa aag gtt tac  ttt gat aaa aat ggt  atc caa gcc      4644
Val Gly  Asn Gln Lys Val Tyr  Phe Asp Lys Asn Gly  Ile Gln Ala          
    1535                 1540                 1545                      

aaa ggt  aag gct gta aga act  tct gat ggt aag gtt  cgc tac ttt      4689
Lys Gly  Lys Ala Val Arg Thr  Ser Asp Gly Lys Val  Arg Tyr Phe          
    1550                 1555                 1560                      

gat gaa  aat tct ggt agc atg  att acc aac caa tgg  aaa ttt gtt      4734
Asp Glu  Asn Ser Gly Ser Met  Ile Thr Asn Gln Trp  Lys Phe Val          
    1565                 1570                 1575                      

tac gga  caa tat tac tat ttc  ggt agt gat ggt gct  gct gtc tac      4779
Tyr Gly  Gln Tyr Tyr Tyr Phe  Gly Ser Asp Gly Ala  Ala Val Tyr          
    1580                 1585                 1590                      

cgt ggc  tgg aac taa                                                4794
Arg Gly  Trp Asn                                                        
    1595                                                                


<210>  2
<211>  1597
<212>  PRT
<213>  Streptococcus downei
<220>
<221>  misc_feature
<222>  (3154)..(4197)
<223>  C-terminal (glucan binding) domain of gene encoding mutansucrase 

<220>
<221>  misc_feature
<222>  (115)..(438)
<223>  variable region of gene encoding mutansucrase

<220>
<221>  misc_feature
<222>  (439)..(3153)
<223>  active core region of gene encoding mutansucrase

<400>  2

Met Glu Lys Asn Glu Arg Phe Lys Met His Lys Val Lys Lys Arg Trp 
1               5                   10                  15      


Val Thr Ile Ser Val Ala Ser Ala Thr Met Leu Ala Ser Ala Leu Gly 
            20                  25                  30          


Ala Ser Val Ala Ser Ala Asp Thr Glu Thr Val Ser Glu Asp Ser Asn 
        35                  40                  45              


Gln Ala Val Leu Thr Ala Asp Gln Thr Thr Thr Asn Gln Asp Thr Glu 
    50                  55                  60                  


Gln Thr Ser Val Ala Ala Thr Ala Thr Ser Glu Gln Ser Ala Ser Thr 
65                  70                  75                  80  


Asp Ala Ala Thr Asp Gln Ala Ser Ala Thr Asp Gln Ala Ser Ala Ala 
                85                  90                  95      


Glu Gln Thr Gln Gly Thr Thr Ala Ser Thr Asp Thr Ala Ala Gln Thr 
            100                 105                 110         


Thr Thr Asn Ala Asn Glu Ala Lys Trp Val Pro Thr Glu Asn Glu Asn 
        115                 120                 125             


Gln Val Phe Thr Asp Glu Met Leu Ala Glu Ala Lys Asn Val Ala Thr 
    130                 135                 140                 


Ala Glu Ser Asn Ser Ile Pro Ser Asp Leu Ala Lys Met Ser Asn Val 
145                 150                 155                 160 


Lys Gln Val Asp Gly Lys Tyr Tyr Tyr Tyr Asp Gln Asp Gly Asn Val 
                165                 170                 175     


Lys Lys Asn Phe Ala Val Ser Val Gly Glu Lys Ile Tyr Tyr Phe Asp 
            180                 185                 190         


Glu Thr Gly Ala Tyr Lys Asp Thr Ser Lys Val Glu Ala Asp Lys Ser 
        195                 200                 205             


Gly Ser Asp Ile Ser Lys Glu Glu Thr Thr Phe Ala Ala Asn Asn Arg 
    210                 215                 220                 


Ala Tyr Ser Thr Ser Ala Glu Asn Phe Glu Ala Ile Asp Asn Tyr Leu 
225                 230                 235                 240 


Thr Ala Asp Ser Trp Tyr Arg Pro Lys Ser Ile Leu Lys Asp Gly Lys 
                245                 250                 255     


Thr Trp Thr Glu Ser Ser Lys Asp Asp Phe Arg Pro Leu Leu Met Ala 
            260                 265                 270         


Trp Trp Pro Asp Thr Glu Thr Lys Arg Asn Tyr Val Asn Tyr Met Asn 
        275                 280                 285             


Lys Val Val Gly Ile Asp Lys Thr Tyr Thr Ala Glu Thr Ser Gln Ala 
    290                 295                 300                 


Asp Leu Thr Ala Ala Ala Glu Leu Val Gln Ala Arg Ile Glu Gln Lys 
305                 310                 315                 320 


Ile Thr Thr Glu Gln Asn Thr Lys Trp Leu Arg Glu Ala Ile Ser Ala 
                325                 330                 335     


Phe Val Lys Thr Gln Pro Gln Trp Asn Gly Glu Ser Glu Lys Pro Tyr 
            340                 345                 350         


Asp Asp His Leu Gln Asn Gly Ala Leu Lys Phe Asp Asn Gln Ser Asp 
        355                 360                 365             


Leu Thr Pro Asp Thr Gln Ser Asn Tyr Arg Leu Leu Asn Arg Thr Pro 
    370                 375                 380                 


Thr Asn Gln Thr Gly Ser Leu Asp Ser Arg Phe Thr Tyr Asn Ala Asn 
385                 390                 395                 400 


Asp Pro Leu Gly Gly Tyr Glu Leu Leu Leu Ala Asn Asp Val Asp Asn 
                405                 410                 415     


Ser Asn Pro Ile Val Gln Ala Glu Gln Leu Asn Trp Leu His Tyr Leu 
            420                 425                 430         


Leu Asn Phe Gly Thr Ile Tyr Ala Lys Asp Ala Asp Ala Asn Phe Asp 
        435                 440                 445             


Ser Ile Arg Val Asp Ala Val Asp Asn Val Asp Ala Asp Leu Leu Gln 
    450                 455                 460                 


Ile Ser Ser Asp Tyr Leu Lys Ala Ala Tyr Gly Ile Asp Lys Asn Asn 
465                 470                 475                 480 


Lys Asn Ala Asn Asn His Val Ser Ile Val Glu Ala Trp Ser Asp Asn 
                485                 490                 495     


Asp Thr Pro Tyr Leu His Asp Asp Gly Asp Asn Leu Met Asn Met Asp 
            500                 505                 510         


Asn Lys Phe Arg Leu Ser Met Leu Trp Ser Leu Ala Lys Pro Leu Asp 
        515                 520                 525             


Lys Arg Ser Gly Leu Asn Pro Leu Ile His Asn Ser Leu Val Asp Arg 
    530                 535                 540                 


Glu Val Asp Asp Arg Glu Val Glu Thr Val Pro Ser Tyr Ser Phe Ala 
545                 550                 555                 560 


Arg Ala His Asp Ser Glu Val Gln Asp Leu Ile Arg Asp Ile Ile Lys 
                565                 570                 575     


Ala Glu Ile Asn Pro Asn Ala Phe Gly Tyr Ser Phe Thr Gln Asp Glu 
            580                 585                 590         


Ile Asp Gln Ala Phe Lys Ile Tyr Asn Glu Asp Leu Lys Lys Thr Asp 
        595                 600                 605             


Lys Lys Tyr Thr His Tyr Asn Val Pro Leu Ser Tyr Thr Leu Leu Leu 
    610                 615                 620                 


Thr Asn Lys Gly Ser Ile Pro Arg Val Tyr Tyr Gly Asp Met Phe Thr 
625                 630                 635                 640 


Asp Asp Gly Gln Tyr Met Ala Asn Lys Thr Val Asn Tyr Asp Ala Ile 
                645                 650                 655     


Glu Ser Leu Leu Lys Ala Arg Met Lys Tyr Val Ala Gly Gly Gln Ala 
            660                 665                 670         


Met Gln Asn Tyr Gln Ile Gly Asn Gly Glu Ile Leu Thr Ser Val Arg 
        675                 680                 685             


Tyr Gly Lys Gly Ala Leu Lys Gln Ser Asp Lys Gly Asp Ala Thr Thr 
    690                 695                 700                 


Arg Thr Ser Gly Val Gly Val Val Met Gly Asn Gln Pro Asn Phe Ser 
705                 710                 715                 720 


Leu Asp Gly Lys Val Val Ala Leu Asn Met Gly Ala Ala His Ala Asn 
                725                 730                 735     


Gln Glu Tyr Arg Ala Leu Met Val Ser Thr Lys Asp Gly Val Ala Thr 
            740                 745                 750         


Tyr Ala Thr Asp Ala Asp Ala Ser Lys Ala Gly Leu Val Lys Arg Thr 
        755                 760                 765             


Asp Glu Asn Gly Tyr Leu Tyr Phe Leu Asn Asp Asp Leu Lys Gly Val 
    770                 775                 780                 


Ala Asn Pro Gln Val Ser Gly Phe Leu Gln Val Trp Val Pro Val Gly 
785                 790                 795                 800 


Ala Ala Asp Asp Gln Asp Ile Arg Val Ala Ala Ser Asp Thr Ala Ser 
                805                 810                 815     


Thr Asp Gly Lys Ser Leu His Gln Asp Ala Ala Met Asp Ser Arg Val 
            820                 825                 830         


Met Phe Glu Gly Phe Ser Asn Phe Gln Ser Phe Ala Thr Lys Glu Glu 
        835                 840                 845             


Glu Tyr Thr Asn Val Val Ile Ala Asn Asn Val Asp Lys Phe Val Ser 
    850                 855                 860                 


Trp Gly Ile Thr Asp Phe Glu Met Ala Pro Gln Tyr Val Ser Ser Thr 
865                 870                 875                 880 


Asp Gly Gln Phe Leu Asp Ser Val Ile Gln Asn Gly Tyr Ala Phe Thr 
                885                 890                 895     


Asp Arg Tyr Asp Leu Gly Met Ser Lys Ala Asn Lys Tyr Gly Thr Ala 
            900                 905                 910         


Asp Gln Leu Val Lys Ala Ile Lys Ala Leu His Ala Lys Gly Leu Lys 
        915                 920                 925             


Val Met Ala Asp Trp Val Pro Asp Gln Met Tyr Thr Phe Pro Lys Gln 
    930                 935                 940                 


Glu Val Val Thr Val Thr Arg Thr Asp Lys Phe Gly Lys Pro Ile Ala 
945                 950                 955                 960 


Gly Ser Gln Ile Asn His Ser Leu Tyr Val Thr Asp Thr Lys Ser Ser 
                965                 970                 975     


Gly Asp Asp Tyr Gln Ala Lys Tyr Gly Gly Ala Phe Leu Asp Glu Leu 
            980                 985                 990         


Lys Glu Lys Tyr Pro Glu Leu Phe  Thr Lys Lys Gln Ile  Ser Thr Gly 
        995                 1000                 1005             


Gln Ala  Ile Asp Pro Ser Val  Lys Ile Lys Gln Trp  Ser Ala Lys 
    1010                 1015                 1020             


Tyr Phe  Asn Gly Ser Asn Ile  Leu Gly Arg Gly Ala  Asp Tyr Val 
    1025                 1030                 1035             


Leu Ser  Asp Gln Ala Ser Asn  Lys Tyr Leu Asn Val  Ser Asp Asp 
    1040                 1045                 1050             


Lys Leu  Phe Leu Pro Lys Thr  Leu Leu Gly Gln Val  Val Glu Ser 
    1055                 1060                 1065             


Gly Ile  Arg Phe Asp Gly Thr  Gly Tyr Val Tyr Asn  Ser Ser Thr 
    1070                 1075                 1080             


Thr Gly  Glu Lys Val Thr Asp  Ser Phe Ile Thr Glu  Ala Gly Asn 
    1085                 1090                 1095             


Leu Tyr  Tyr Phe Gly Gln Asp  Gly Tyr Met Val Thr  Gly Ala Gln 
    1100                 1105                 1110             


Asn Ile  Lys Gly Ser Asn Tyr  Tyr Phe Leu Ala Asn  Gly Ala Ala 
    1115                 1120                 1125             


Leu Arg  Asn Thr Val Tyr Thr  Asp Ala Gln Gly Gln  Asn His Tyr 
    1130                 1135                 1140             


Tyr Gly  Asn Asp Gly Lys Arg  Tyr Glu Asn Gly Tyr  Gln Gln Phe 
    1145                 1150                 1155             


Gly Asn  Asp Ser Trp Arg Tyr  Phe Lys Asn Gly Val  Met Ala Leu 
    1160                 1165                 1170             


Gly Leu  Thr Thr Val Asp Gly  His Val Gln Tyr Phe  Asp Lys Asp 
    1175                 1180                 1185             


Gly Val  Gln Ala Lys Asp Lys  Ile Ile Val Thr Arg  Asp Gly Lys 
    1190                 1195                 1200             


Val Arg  Tyr Phe Asp Gln His  Asn Gly Asn Ala Val  Thr Asn Thr 
    1205                 1210                 1215             


Phe Val  Ala Asp Lys Thr Gly  His Trp Tyr Tyr Leu  Gly Lys Asp 
    1220                 1225                 1230             


Gly Val  Ala Val Thr Gly Ala  Gln Thr Val Gly Lys  Gln His Leu 
    1235                 1240                 1245             


Tyr Phe  Glu Ala Asn Gly Gln  Gln Val Lys Gly Asp  Phe Val Thr 
    1250                 1255                 1260             


Ala Lys  Asp Gly Lys Leu Tyr  Phe Tyr Asp Val Asp  Ser Gly Asp 
    1265                 1270                 1275             


Met Trp  Thr Asn Thr Phe Ile  Glu Asp Lys Ala Gly  Asn Trp Phe 
    1280                 1285                 1290             


Tyr Leu  Gly Lys Asp Gly Ala  Ala Val Thr Gly Ala  Gln Thr Ile 
    1295                 1300                 1305             


Lys Gly  Gln Lys Leu Tyr Phe  Lys Ala Asn Gly Gln  Gln Val Lys 
    1310                 1315                 1320             


Gly Asp  Ile Val Lys Asp Ala  Asp Gly Lys Ile Arg  Tyr Tyr Asp 
    1325                 1330                 1335             


Ala Gln  Thr Gly Glu Gln Val  Phe Asn Lys Ser Val  Ser Val Asn 
    1340                 1345                 1350             


Gly Lys  Thr Tyr Tyr Phe Gly  Ser Asp Gly Thr Ala  Gln Thr Gln 
    1355                 1360                 1365             


Ala Asn  Pro Lys Gly Gln Thr  Phe Lys Asp Gly Ser  Gly Val Leu 
    1370                 1375                 1380             


Arg Phe  Tyr Asn Leu Glu Gly  Gln Tyr Val Ser Gly  Ser Gly Trp 
    1385                 1390                 1395             


Tyr Glu  Thr Ala Glu His Glu  Trp Val Tyr Val Lys  Ser Gly Lys 
    1400                 1405                 1410             


Val Leu  Thr Gly Ala Gln Thr  Ile Gly Asn Gln Arg  Val Tyr Phe 
    1415                 1420                 1425             


Lys Asp  Asn Gly His Gln Val  Lys Gly Gln Leu Val  Thr Gly Asn 
    1430                 1435                 1440             


Asp Gly  Lys Leu Arg Tyr Tyr  Asp Ala Asn Ser Gly  Asp Gln Ala 
    1445                 1450                 1455             


Phe Asn  Lys Ser Val Thr Val  Asn Gly Lys Thr Tyr  Tyr Phe Gly 
    1460                 1465                 1470             


Ser Asp  Gly Thr Ala Gln Thr  Gln Ala Asn Pro Lys  Gly Gln Thr 
    1475                 1480                 1485             


Phe Lys  Asp Gly Ser Gly Val  Leu Arg Phe Tyr Asn  Leu Glu Gly 
    1490                 1495                 1500             


Gln Tyr  Val Ser Gly Ser Gly  Trp Tyr Lys Asn Ala  Gln Gly Gln 
    1505                 1510                 1515             


Trp Leu  Tyr Val Lys Asp Gly  Lys Val Leu Thr Gly  Leu Gln Thr 
    1520                 1525                 1530             


Val Gly  Asn Gln Lys Val Tyr  Phe Asp Lys Asn Gly  Ile Gln Ala 
    1535                 1540                 1545             


Lys Gly  Lys Ala Val Arg Thr  Ser Asp Gly Lys Val  Arg Tyr Phe 
    1550                 1555                 1560             


Asp Glu  Asn Ser Gly Ser Met  Ile Thr Asn Gln Trp  Lys Phe Val 
    1565                 1570                 1575             


Tyr Gly  Gln Tyr Tyr Tyr Phe  Gly Ser Asp Gly Ala  Ala Val Tyr 
    1580                 1585                 1590             


Arg Gly  Trp Asn 
    1595         


<210>  3
<211>  3039<
212>  DNA
<213>  Streptococcus downei
<220>
<221>  CDS
<222>  (1)..(3036)
<223>  variable region plus active core region of mutansucrase gene

<220>
<221>  misc_feature
<222>  (4)..(324)
<223>  variable region of mutansucrase gene 

<220>
<221>  active core region
<222>  (325)..(3036)
<223>  active core region of mutansucrase gene

<220>
<221>  mutation
<222>  (3038)..(3038)
<223>  C to A

<220>
<221>  mutation
<222>  (1)..(3)
<223>  GAT to GGG

<400>  3
ggg act gaa act gtt agc gaa gac agc aac caa gca gtc ttg acg gct       48
Gly Thr Glu Thr Val Ser Glu Asp Ser Asn Gln Ala Val Leu Thr Ala         
1               5                   10                  15              

gac caa acg act acc aac caa gat act gag caa act tct gtt gca gcg       96
Asp Gln Thr Thr Thr Asn Gln Asp Thr Glu Gln Thr Ser Val Ala Ala         
            20                  25                  30                  

aca gct aca tca gaa cag tct gct tca act gat gca gca aca gat caa      144
Thr Ala Thr Ser Glu Gln Ser Ala Ser Thr Asp Ala Ala Thr Asp Gln         
        35                  40                  45                      

gca tca gca aca gat caa gca tca gca gca gag caa act caa gga aca      192
Ala Ser Ala Thr Asp Gln Ala Ser Ala Ala Glu Gln Thr Gln Gly Thr         
    50                  55                  60                          

aca gct agc aca gac acg gca gct caa aca acc aca aat gct aat gaa      240
Thr Ala Ser Thr Asp Thr Ala Ala Gln Thr Thr Thr Asn Ala Asn Glu         
65                  70                  75                  80          

gct aag tgg gtt ccg act gaa aat gag aac caa gtt ttt aca gat gag      288
Ala Lys Trp Val Pro Thr Glu Asn Glu Asn Gln Val Phe Thr Asp Glu         
                85                  90                  95              

atg tta gca gaa gcc aag aat gtg gct act gct gaa tct aat tca att      336
Met Leu Ala Glu Ala Lys Asn Val Ala Thr Ala Glu Ser Asn Ser Ile         
            100                 105                 110                 

cca tca gac ttg gct aaa atg tca aat gtt aag cag gtt gac ggt aaa      384
Pro Ser Asp Leu Ala Lys Met Ser Asn Val Lys Gln Val Asp Gly Lys         
        115                 120                 125                     

tat tat tac tac gat caa gac ggc aac gtt aag aag aac ttt gct gtt      432
Tyr Tyr Tyr Tyr Asp Gln Asp Gly Asn Val Lys Lys Asn Phe Ala Val         
    130                 135                 140                         

agc gtt ggt gag aag atc tat tac ttt gat gaa act ggc gct tac aaa      480
Ser Val Gly Glu Lys Ile Tyr Tyr Phe Asp Glu Thr Gly Ala Tyr Lys         
145                 150                 155                 160         

gac act agc aag gta gaa gcg gat aaa tca ggt tct gat atc agc aaa      528
Asp Thr Ser Lys Val Glu Ala Asp Lys Ser Gly Ser Asp Ile Ser Lys         
                165                 170                 175             

gaa gaa aca acc ttt gct gct aac aac cgt gct tac agt acc tca gct      576
Glu Glu Thr Thr Phe Ala Ala Asn Asn Arg Ala Tyr Ser Thr Ser Ala         
            180                 185                 190                 

gaa aac ttt gaa gcc att gat aac tac ttg aca gct gac tca tgg tac      624
Glu Asn Phe Glu Ala Ile Asp Asn Tyr Leu Thr Ala Asp Ser Trp Tyr         
        195                 200                 205                     

cgt cca aag tct atc ctc aag gat ggt aag act tgg aca gaa tca agc      672
Arg Pro Lys Ser Ile Leu Lys Asp Gly Lys Thr Trp Thr Glu Ser Ser         
    210                 215                 220                         

aag gat gac ttc cgt ccg cta ttg atg gct tgg tgg cca gat acc gaa      720
Lys Asp Asp Phe Arg Pro Leu Leu Met Ala Trp Trp Pro Asp Thr Glu         
225                 230                 235                 240         

acc aaa cgc aac tat gtt aac tac atg aac aag gtt gtt ggt atc gat      768
Thr Lys Arg Asn Tyr Val Asn Tyr Met Asn Lys Val Val Gly Ile Asp         
                245                 250                 255             

aaa acc tat acc gct gaa aca agc cag gct gac ctg aca gca gca gct      816
Lys Thr Tyr Thr Ala Glu Thr Ser Gln Ala Asp Leu Thr Ala Ala Ala         
            260                 265                 270                 

gaa ctg gtt caa gcc cgt atc gaa caa aag att aca act gaa caa aat      864
Glu Leu Val Gln Ala Arg Ile Glu Gln Lys Ile Thr Thr Glu Gln Asn         
        275                 280                 285                     

acc aaa tgg ttg cgt gaa gct atc tca gct ttt gtt aaa act caa cca      912
Thr Lys Trp Leu Arg Glu Ala Ile Ser Ala Phe Val Lys Thr Gln Pro         
    290                 295                 300                         

caa tgg aat ggt gaa agc gaa aag cca tac gat gac cac ttg caa aat      960
Gln Trp Asn Gly Glu Ser Glu Lys Pro Tyr Asp Asp His Leu Gln Asn         
305                 310                 315                 320         

ggt gcc ctc aag ttc gat aac caa tct gat ttg aca cca gat acg caa     1008
Gly Ala Leu Lys Phe Asp Asn Gln Ser Asp Leu Thr Pro Asp Thr Gln         
                325                 330                 335             

tcg aac tat cgt ttg ctc aat cgc aca cca act aac caa act ggt tcc     1056
Ser Asn Tyr Arg Leu Leu Asn Arg Thr Pro Thr Asn Gln Thr Gly Ser         
            340                 345                 350                 

ctg gac tct cgt ttc acc tac aat gct aac gac ccg tta ggt ggt tat     1104
Leu Asp Ser Arg Phe Thr Tyr Asn Ala Asn Asp Pro Leu Gly Gly Tyr         
        355                 360                 365                     

gag ttg ctt ctg gct aac gac gtg gat aac tct aat ccc atc gtt caa     1152
Glu Leu Leu Leu Ala Asn Asp Val Asp Asn Ser Asn Pro Ile Val Gln         
    370                 375                 380                         

gca gag caa ctc aac tgg ctg cat tac ctg ctc aac ttc ggt act atc     1200
Ala Glu Gln Leu Asn Trp Leu His Tyr Leu Leu Asn Phe Gly Thr Ile         
385                 390                 395                 400         

tat gct aaa gat gcg gat gct aac ttt gac tct atc cgt gtt gat gcg     1248
Tyr Ala Lys Asp Ala Asp Ala Asn Phe Asp Ser Ile Arg Val Asp Ala         
                405                 410                 415             

gta gat aat gtc gat gct gac ctt ctg caa atc tct agt gat tac ctt     1296
Val Asp Asn Val Asp Ala Asp Leu Leu Gln Ile Ser Ser Asp Tyr Leu         
            420                 425                 430                 

aag gca gct tac ggt atc gat aaa aac aac aaa aat gct aat aac cac     1344
Lys Ala Ala Tyr Gly Ile Asp Lys Asn Asn Lys Asn Ala Asn Asn His         
        435                 440                 445                     

gtt tct atc gta gaa gca tgg agc gac aac gat acc cct tat ctc cat     1392
Val Ser Ile Val Glu Ala Trp Ser Asp Asn Asp Thr Pro Tyr Leu His         
    450                 455                 460                         

gat gat ggc gac aac ctc atg aac atg gac aac aag ttc cgt ttg tct     1440
Asp Asp Gly Asp Asn Leu Met Asn Met Asp Asn Lys Phe Arg Leu Ser         
465                 470                 475                 480         

atg ctt tgg tct ttg gct aaa cca ttg gac aaa cgt tct ggc ttg aat     1488
Met Leu Trp Ser Leu Ala Lys Pro Leu Asp Lys Arg Ser Gly Leu Asn         
                485                 490                 495             

ccc ctc atc cac aac agt ctg gtt gac cgt gaa gtg gat gac cgt gaa     1536
Pro Leu Ile His Asn Ser Leu Val Asp Arg Glu Val Asp Asp Arg Glu         
            500                 505                 510                 

gtt gaa acc gtt cca agt tac agc ttt gcc cgt gct cac gat agc gaa     1584
Val Glu Thr Val Pro Ser Tyr Ser Phe Ala Arg Ala His Asp Ser Glu         
        515                 520                 525                     

gta caa gac ctg att cgt gac atc atc aag gct gaa att aat cca aat     1632
Val Gln Asp Leu Ile Arg Asp Ile Ile Lys Ala Glu Ile Asn Pro Asn         
    530                 535                 540                         

gca ttt ggt tat tca ttt act caa gac gaa atc gac caa gcc ttc aag     1680
Ala Phe Gly Tyr Ser Phe Thr Gln Asp Glu Ile Asp Gln Ala Phe Lys         
545                 550                 555                 560         

att tac aac gaa gac ctc aag aag acc gat aag aaa tac act cac tac     1728
Ile Tyr Asn Glu Asp Leu Lys Lys Thr Asp Lys Lys Tyr Thr His Tyr         
                565                 570                 575             

aat gtg ccg ctt tct tat acc ttg ctt ctg act aac aag ggt tcg att     1776
Asn Val Pro Leu Ser Tyr Thr Leu Leu Leu Thr Asn Lys Gly Ser Ile         
            580                 585                 590                 

cct cgc gtc tat tat gga gat atg ttc acc gat gat ggt caa tac atg     1824
Pro Arg Val Tyr Tyr Gly Asp Met Phe Thr Asp Asp Gly Gln Tyr Met         
        595                 600                 605                     

gcc aac aag act gtg aac tac gat gct atc gaa tct ctg ctg aaa gcc     1872
Ala Asn Lys Thr Val Asn Tyr Asp Ala Ile Glu Ser Leu Leu Lys Ala         
    610                 615                 620                         

cgt atg aag tac gtt gct ggt ggt caa gct atg caa aat tac caa atc     1920
Arg Met Lys Tyr Val Ala Gly Gly Gln Ala Met Gln Asn Tyr Gln Ile         
625                 630                 635                 640         

ggt aat ggc gaa atc ttg act tct gtc cgt tat ggt aag ggt gcc ctt     1968
Gly Asn Gly Glu Ile Leu Thr Ser Val Arg Tyr Gly Lys Gly Ala Leu         
                645                 650                 655             

aaa caa agc gat aag ggt gat gcg aca act cgt acg tca ggt gtc ggc     2016
Lys Gln Ser Asp Lys Gly Asp Ala Thr Thr Arg Thr Ser Gly Val Gly         
            660                 665                 670                 

gtt gtt atg gga aac caa ccc aac ttt agc ttg gat gga aag gtt gta     2064
Val Val Met Gly Asn Gln Pro Asn Phe Ser Leu Asp Gly Lys Val Val         
        675                 680                 685                     

gcc ctc aac atg ggt gct gcc cac gct aac caa gaa tac cgt gct ctt     2112
Ala Leu Asn Met Gly Ala Ala His Ala Asn Gln Glu Tyr Arg Ala Leu         
    690                 695                 700                         

atg gta tca act aaa gac ggt gtt gca acc tat gct aca gat gct gat     2160
Met Val Ser Thr Lys Asp Gly Val Ala Thr Tyr Ala Thr Asp Ala Asp         
705                 710                 715                 720         

gct agc aag gct ggt ctg gtt aag cgc aca gat gaa aat ggt tac ctc     2208
Ala Ser Lys Ala Gly Leu Val Lys Arg Thr Asp Glu Asn Gly Tyr Leu         
                725                 730                 735             

tac ttc ttg aac gac gat ctc aag ggg gtt gct aac cct cag gtt tct     2256
Tyr Phe Leu Asn Asp Asp Leu Lys Gly Val Ala Asn Pro Gln Val Ser         
            740                 745                 750                 

ggt ttc ctt caa gtc tgg gta cca gtg gga gca gca gat gac caa gat     2304
Gly Phe Leu Gln Val Trp Val Pro Val Gly Ala Ala Asp Asp Gln Asp         
        755                 760                 765                     

att cgt gta gca gct agc gat aca gca agt acc gat gga aaa tca ctc     2352
Ile Arg Val Ala Ala Ser Asp Thr Ala Ser Thr Asp Gly Lys Ser Leu         
    770                 775                 780                         

cat caa gat gct gcc atg gac tct cgc gtc atg ttt gaa ggt ttc tct     2400
His Gln Asp Ala Ala Met Asp Ser Arg Val Met Phe Glu Gly Phe Ser         
785                 790                 795                 800         

aac ttc caa tct ttt gcg aca aaa gaa gaa gag tat acc aat gtt gtt     2448
Asn Phe Gln Ser Phe Ala Thr Lys Glu Glu Glu Tyr Thr Asn Val Val         
                805                 810                 815             

att gct aac aat gtt gat aaa ttt gtt tca tgg gga atc act gac ttt     2496
Ile Ala Asn Asn Val Asp Lys Phe Val Ser Trp Gly Ile Thr Asp Phe         
            820                 825                 830                 

gaa atg gct cct cag tat gtc tca tct act gac ggt cag ttc ctt gat     2544
Glu Met Ala Pro Gln Tyr Val Ser Ser Thr Asp Gly Gln Phe Leu Asp         
        835                 840                 845                     

tct gtc att caa aat ggt tat gcc ttt acc gac cgt tat gac ttg ggt     2592
Ser Val Ile Gln Asn Gly Tyr Ala Phe Thr Asp Arg Tyr Asp Leu Gly         
    850                 855                 860                         

atg tct aaa gca aac aag tat ggt aca gcc gac caa ttg gtt aag gct     2640
Met Ser Lys Ala Asn Lys Tyr Gly Thr Ala Asp Gln Leu Val Lys Ala         
865                 870                 875                 880         

atc aag gct ctc cat gct aaa ggc ctg aag gtt atg gca gac tgg gtt     2688
Ile Lys Ala Leu His Ala Lys Gly Leu Lys Val Met Ala Asp Trp Val         
                885                 890                 895             

cca gac caa atg tac acc ttc cct aaa caa gaa gtg gtc act gtt act     2736
Pro Asp Gln Met Tyr Thr Phe Pro Lys Gln Glu Val Val Thr Val Thr         
            900                 905                 910                 

cgg aca gat aag ttt ggc aaa cca atc gca gga agc caa att aat cac     2784
Arg Thr Asp Lys Phe Gly Lys Pro Ile Ala Gly Ser Gln Ile Asn His         
        915                 920                 925                     

agt ctc tac gta aca gat aca aag agc tct ggt gat gac tat caa gct     2832
Ser Leu Tyr Val Thr Asp Thr Lys Ser Ser Gly Asp Asp Tyr Gln Ala         
    930                 935                 940                         

aaa tac ggc ggt gcc ttc ctt gac gaa tta aag gaa aaa tat cca gaa     2880
Lys Tyr Gly Gly Ala Phe Leu Asp Glu Leu Lys Glu Lys Tyr Pro Glu         
945                 950                 955                 960         

ctc ttt acc aag aag caa atc tct acc ggc caa gcc ata gat cca tct     2928
Leu Phe Thr Lys Lys Gln Ile Ser Thr Gly Gln Ala Ile Asp Pro Ser         
                965                 970                 975             

gtt aag att aaa caa tgg tct gct aag tac ttc aac ggt agc aat atc     2976
Val Lys Ile Lys Gln Trp Ser Ala Lys Tyr Phe Asn Gly Ser Asn Ile         
            980                 985                 990                 

ctt ggc cgg ggt gcc gat tat gtc  ctc agc gac caa gca  agc aac aag   3024
Leu Gly Arg Gly Ala Asp Tyr Val  Leu Ser Asp Gln Ala  Ser Asn Lys       
        995                 1000                 1005                   

tac ctc  aat gtt taa                                                3039
Tyr Leu  Asn Val                                                        
    1010                                                                


<210>  4
<211>  1012
<212>  PRT
<213>  Streptococcus downei
<220>
<221>  misc_feature
<222>  (4)..(324)
<223>  variable region of mutansucrase gene 

<400>  4

Gly Thr Glu Thr Val Ser Glu Asp Ser Asn Gln Ala Val Leu Thr Ala 
1               5                   10                  15      


Asp Gln Thr Thr Thr Asn Gln Asp Thr Glu Gln Thr Ser Val Ala Ala 
            20                  25                  30          


Thr Ala Thr Ser Glu Gln Ser Ala Ser Thr Asp Ala Ala Thr Asp Gln 
        35                  40                  45              


Ala Ser Ala Thr Asp Gln Ala Ser Ala Ala Glu Gln Thr Gln Gly Thr 
    50                  55                  60                  


Thr Ala Ser Thr Asp Thr Ala Ala Gln Thr Thr Thr Asn Ala Asn Glu 
65                  70                  75                  80  


Ala Lys Trp Val Pro Thr Glu Asn Glu Asn Gln Val Phe Thr Asp Glu 
                85                  90                  95      


Met Leu Ala Glu Ala Lys Asn Val Ala Thr Ala Glu Ser Asn Ser Ile 
            100                 105                 110         


Pro Ser Asp Leu Ala Lys Met Ser Asn Val Lys Gln Val Asp Gly Lys 
        115                 120                 125             


Tyr Tyr Tyr Tyr Asp Gln Asp Gly Asn Val Lys Lys Asn Phe Ala Val 
    130                 135                 140                 


Ser Val Gly Glu Lys Ile Tyr Tyr Phe Asp Glu Thr Gly Ala Tyr Lys 
145                 150                 155                 160 


Asp Thr Ser Lys Val Glu Ala Asp Lys Ser Gly Ser Asp Ile Ser Lys 
                165                 170                 175     


Glu Glu Thr Thr Phe Ala Ala Asn Asn Arg Ala Tyr Ser Thr Ser Ala 
            180                 185                 190         


Glu Asn Phe Glu Ala Ile Asp Asn Tyr Leu Thr Ala Asp Ser Trp Tyr 
        195                 200                 205             


Arg Pro Lys Ser Ile Leu Lys Asp Gly Lys Thr Trp Thr Glu Ser Ser 
    210                 215                 220                 


Lys Asp Asp Phe Arg Pro Leu Leu Met Ala Trp Trp Pro Asp Thr Glu 
225                 230                 235                 240 


Thr Lys Arg Asn Tyr Val Asn Tyr Met Asn Lys Val Val Gly Ile Asp 
                245                 250                 255     


Lys Thr Tyr Thr Ala Glu Thr Ser Gln Ala Asp Leu Thr Ala Ala Ala 
            260                 265                 270         


Glu Leu Val Gln Ala Arg Ile Glu Gln Lys Ile Thr Thr Glu Gln Asn 
        275                 280                 285             


Thr Lys Trp Leu Arg Glu Ala Ile Ser Ala Phe Val Lys Thr Gln Pro 
    290                 295                 300                 


Gln Trp Asn Gly Glu Ser Glu Lys Pro Tyr Asp Asp His Leu Gln Asn 
305                 310                 315                 320 


Gly Ala Leu Lys Phe Asp Asn Gln Ser Asp Leu Thr Pro Asp Thr Gln 
                325                 330                 335     


Ser Asn Tyr Arg Leu Leu Asn Arg Thr Pro Thr Asn Gln Thr Gly Ser 
            340                 345                 350         


Leu Asp Ser Arg Phe Thr Tyr Asn Ala Asn Asp Pro Leu Gly Gly Tyr 
        355                 360                 365             


Glu Leu Leu Leu Ala Asn Asp Val Asp Asn Ser Asn Pro Ile Val Gln 
    370                 375                 380                 


Ala Glu Gln Leu Asn Trp Leu His Tyr Leu Leu Asn Phe Gly Thr Ile 
385                 390                 395                 400 


Tyr Ala Lys Asp Ala Asp Ala Asn Phe Asp Ser Ile Arg Val Asp Ala 
                405                 410                 415     


Val Asp Asn Val Asp Ala Asp Leu Leu Gln Ile Ser Ser Asp Tyr Leu 
            420                 425                 430         


Lys Ala Ala Tyr Gly Ile Asp Lys Asn Asn Lys Asn Ala Asn Asn His 
        435                 440                 445             


Val Ser Ile Val Glu Ala Trp Ser Asp Asn Asp Thr Pro Tyr Leu His 
    450                 455                 460                 


Asp Asp Gly Asp Asn Leu Met Asn Met Asp Asn Lys Phe Arg Leu Ser 
465                 470                 475                 480 


Met Leu Trp Ser Leu Ala Lys Pro Leu Asp Lys Arg Ser Gly Leu Asn 
                485                 490                 495     


Pro Leu Ile His Asn Ser Leu Val Asp Arg Glu Val Asp Asp Arg Glu 
            500                 505                 510         


Val Glu Thr Val Pro Ser Tyr Ser Phe Ala Arg Ala His Asp Ser Glu 
        515                 520                 525             


Val Gln Asp Leu Ile Arg Asp Ile Ile Lys Ala Glu Ile Asn Pro Asn 
    530                 535                 540                 


Ala Phe Gly Tyr Ser Phe Thr Gln Asp Glu Ile Asp Gln Ala Phe Lys 
545                 550                 555                 560 


Ile Tyr Asn Glu Asp Leu Lys Lys Thr Asp Lys Lys Tyr Thr His Tyr 
                565                 570                 575     


Asn Val Pro Leu Ser Tyr Thr Leu Leu Leu Thr Asn Lys Gly Ser Ile 
            580                 585                 590         


Pro Arg Val Tyr Tyr Gly Asp Met Phe Thr Asp Asp Gly Gln Tyr Met 
        595                 600                 605             


Ala Asn Lys Thr Val Asn Tyr Asp Ala Ile Glu Ser Leu Leu Lys Ala 
    610                 615                 620                 


Arg Met Lys Tyr Val Ala Gly Gly Gln Ala Met Gln Asn Tyr Gln Ile 
625                 630                 635                 640 


Gly Asn Gly Glu Ile Leu Thr Ser Val Arg Tyr Gly Lys Gly Ala Leu 
                645                 650                 655     


Lys Gln Ser Asp Lys Gly Asp Ala Thr Thr Arg Thr Ser Gly Val Gly 
            660                 665                 670         


Val Val Met Gly Asn Gln Pro Asn Phe Ser Leu Asp Gly Lys Val Val 
        675                 680                 685             


Ala Leu Asn Met Gly Ala Ala His Ala Asn Gln Glu Tyr Arg Ala Leu 
    690                 695                 700                 


Met Val Ser Thr Lys Asp Gly Val Ala Thr Tyr Ala Thr Asp Ala Asp 
705                 710                 715                 720 


Ala Ser Lys Ala Gly Leu Val Lys Arg Thr Asp Glu Asn Gly Tyr Leu 
                725                 730                 735     


Tyr Phe Leu Asn Asp Asp Leu Lys Gly Val Ala Asn Pro Gln Val Ser 
            740                 745                 750         


Gly Phe Leu Gln Val Trp Val Pro Val Gly Ala Ala Asp Asp Gln Asp 
        755                 760                 765             


Ile Arg Val Ala Ala Ser Asp Thr Ala Ser Thr Asp Gly Lys Ser Leu 
    770                 775                 780                 


His Gln Asp Ala Ala Met Asp Ser Arg Val Met Phe Glu Gly Phe Ser 
785                 790                 795                 800 


Asn Phe Gln Ser Phe Ala Thr Lys Glu Glu Glu Tyr Thr Asn Val Val 
                805                 810                 815     


Ile Ala Asn Asn Val Asp Lys Phe Val Ser Trp Gly Ile Thr Asp Phe 
            820                 825                 830         


Glu Met Ala Pro Gln Tyr Val Ser Ser Thr Asp Gly Gln Phe Leu Asp 
        835                 840                 845             


Ser Val Ile Gln Asn Gly Tyr Ala Phe Thr Asp Arg Tyr Asp Leu Gly 
    850                 855                 860                 


Met Ser Lys Ala Asn Lys Tyr Gly Thr Ala Asp Gln Leu Val Lys Ala 
865                 870                 875                 880 


Ile Lys Ala Leu His Ala Lys Gly Leu Lys Val Met Ala Asp Trp Val 
                885                 890                 895     


Pro Asp Gln Met Tyr Thr Phe Pro Lys Gln Glu Val Val Thr Val Thr 
            900                 905                 910         


Arg Thr Asp Lys Phe Gly Lys Pro Ile Ala Gly Ser Gln Ile Asn His 
        915                 920                 925             


Ser Leu Tyr Val Thr Asp Thr Lys Ser Ser Gly Asp Asp Tyr Gln Ala 
    930                 935                 940                 


Lys Tyr Gly Gly Ala Phe Leu Asp Glu Leu Lys Glu Lys Tyr Pro Glu 
945                 950                 955                 960 


Leu Phe Thr Lys Lys Gln Ile Ser Thr Gly Gln Ala Ile Asp Pro Ser 
                965                 970                 975     


Val Lys Ile Lys Gln Trp Ser Ala Lys Tyr Phe Asn Gly Ser Asn Ile 
            980                 985                 990         


Leu Gly Arg Gly Ala Asp Tyr Val  Leu Ser Asp Gln Ala  Ser Asn Lys 
        995                 1000                 1005             


Tyr Leu  Asn Val 
    1010         


